Effects ofa Combi nation of Activin A and Follistatin
Cleavage rates of presumptive zygotes cultured in mSOF alone (control) and in mSOF containing 10 ng/ml activin A, 10 ng/ml follistatin, or both (10 ng/ml activin A + 10 ng/ml follistatin; 10 ng/ml activin A + 100 ng/ml folli-statin) were all in the range 85-88% and were not significantly different. The percentages of zygotes that developed to the morula and blastocyst stages are shown in Figure 3. As was found in the previous experiment, the percentages of zygotes that developed to the morula and blastocyst stages with addition of 10 ng/ml activin A alone (46% and 32%, respectively) were significantly higher (p < 0.01) than the percentages obtained in the control (38% and 24%, respectively). buy cheap antibiotics
Also, with addition of 10 ng/ml follistatin alone, these percentages (29% and 13%, respectively) were significantly impeded (p < 0.01). In the culture with 10 ng/ml of activin A, supplementation with the same concentration of follistatin reduced the percentages of embryos that developed to morulae and blastocysts to values (41% and 24%, respectively) that were similar to those for the control.