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Bronchial Aspirates in the Diagnosis of Pulmonary Tuberculosis: Data Analysis

Bronchial Aspirates in the Diagnosis of Pulmonary Tuberculosis: Data AnalysisAfter the magnetic particles are washed to remove unbound material, avidin-horseradish peroxidase conjugate binds to the biotin-labeled amplicon captured by the specific DNA probe. After removing unbound conjugates by washing, substrate is added. Specimens with A660 > 0.35 are interpreted as positive for M tuberculosis. Specimens with A660 < 0.35, and internal controls with A660 > 0.35, are interpreted as negative for M tuberculosis.
After CA results were obtained, each patient’s clinical and laboratory records were reviewed, together with chest radiography add comment buy birthcontrol online. The diagnosis of tuberculosis was confirmed only when cultures of sputum or bronchial aspirates were positive for M tuberculosis, or if lung or bronchial biopsy specimens showed chronic granulomatous inflammation with caseation necrosis. Clinical tuberculosis was defined as radiologic findings compatible with tuberculosis, which improved with antituberculosis drug treatment, without bacteriological or histologic confirmation of tuberculosis. In cases of clinical tuberculosis, with histologic evidence of chronic granulomatous inflammation without caseation necrosis, tuberculosis was considered to be confirmed. The sensitivity, specificity, positive predictive value (PPV), and negative predictive value (NPV) of CA testing were calculated, using bacteriologic or histologic diagnoses as the ultimate diagnostic standard for tuberculosis. In the case of patients with false-positive CA results, the clinical history and radiographic results were investigated. Bacteriologic and CA results of the patients who underwent bronchoscopy using the same bronchoscope immediately before the patient with a false-positive CA result were reviewed to investigate the possibility of contamination via the bronchoscopes. Bacteriologic and CA results of the patients in whom the bronchoscope was washed immediately before the patients who underwent bronchoscopy using the same bronchoscopy immediately before the patients with false-positive CA result were reviewed to investigate the possibility of cross-contamination via the bronchoscope washer.