Loading

wait a moment

Bronchial Aspirates in the Diagnosis of Pulmonary Tuberculosis: Sample Preparation

Bronchial Aspirates in the Diagnosis of Pulmonary Tuberculosis: Sample PreparationThe bronchoscope was subsequently disassembled into component parts in preparation for disinfection, and the lumen cleaned manually by brushing with povidone-iodine (Betadine; Mundipharma; Seoul, Korea). The parts were cleaned using the standard protocol. A total of 23 min of automated washing was performed between uses, consisting of a cleaning cycle, a disinfection cycle, and a drying cycle. Disinfection was performed using 2% glutaraldehyde (Cidex; Johnson and Johnson K.K.; Tokyo, Japan) for 10 min. After completion of the broncho-scopic procedures for the day, each of the bronchoscopes was washed for 43 min, including a 23-min disinfection cycle with 2% glutaraldehyde. The name of each bronchoscope, the order of bronchoscopic procedures undertaken each day, and the order of washing during this study period (cumulative numbers 1 to 400) were recorded.
All bronchoscopy was performed by experienced bronchosco-pists, by a transoral or transnasal route so canadian neighborhood pharmacy . Patients were usually premedicated with IM atropine and pethidine. Lidocaine was used for topical anesthesia of the larynx and tracheobronchial trees. After examination of the bronchial trees, 5 to 10 mL of normal saline solution was instilled into the orifice of the bronchial segment in which abnormalities were likely to be located, and then aspirated back. In the cases of patients in whom the abnormalities were not confirmed, normal saline solution was instilled into both bronchial trees. In cases where mucosal abnormalities were detected or lung tissue was required, tissue was obtained using a biopsy forceps.
At least 5 mL of bronchial aspirate and sputum were collected in a sterile conical tube, and then treated with NALC-NaOH. After Ziehl-Neelson staining, 300 fields were examined using light microscopy at high power (X 1,000) by experienced personnel.