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Bronchial Aspirates in the Diagnosis of Pulmonary Tuberculosis: Tuberculosis patients

Bronchial Aspirates in the Diagnosis of Pulmonary Tuberculosis: Tuberculosis patientsHowever, because bronchoscopy is usually required in cases of smear-negative, low-colony-count tuberculosis, it seems that the patient population in this study can be compared to actual clinical situations we meet in everyday practice.
Of 13 smear-positive tuberculosis patients, 5 patients had positive findings in bronchial aspirates but not in sputum. Of 30 tuberculosis patients with negative results for AFB smears on sputum or bronchial aspirates, 12 patients (40%) had positive findings for M tuberculosis on CA tests. Buy claritin online More info These data suggest that AFB smears and CA tests on bronchoscopic aspirates are useful for an earlier diagnosis of sputum smear-negative patients. In the study by Wong et al, the sensitivity of PCR on bronchial aspirate specimens in the diagnosis of smear-negative pulmonary tuberculosis was 97.2%. However, these researchers used in-house PCR assays, and false-positive rates were as high as 27% (22 of 82 cases). They did not mention why the false-positive rate was so high. It may have been due to crosscontamination via bronchoscopes, or to poor quality control. In-house PCR assays usually vary greatly between institutions in both sensitivity and specificity. There is no worldwide standardization, and it may be personnel dependent. For this reason, we selected the CA test to amplify M tuberculosis DNA in this study. The CA test, which automates amplification and detection and decreases hands-on labor, was approved by the US Food and Drug Administration. Others have reported the sensitivity of PCR on BAL specimens to be from 40 to 79%. Had we used BAL fluid instead of bronchial aspirates, the sensitivity may have been improved. However, in this study, we do not know whether the CA test on bronchial aspirates has some advantages over the test done on sputum specimens.