Precursors of other cell lineages or undifferentiated phenotypes likely compose the majority of the cell population within the early EBs used in NPC generation. Therefore, a lower rate of neuronal differentiation would be expected after expansion and differentiation. This is an active area of study along with the characterization of individual ES cell lines and their ability to differentiate into neural lineages. Levitra professional Canadian pharmacy
Two NPC forms were observed during the expansion stage. One type grew as spherical structures, which may be analogous to what others have referred to as neurospheres, and a second grew as an adherent monolayer. We noted that both forms of NPCs can be proliferated in vitro and that both can give rise to differentiated phenotypes. The importance of these findings is now under investigation. In the context of cell-based therapy, in which large numbers of cells may be required to treat acute conditions over relatively short time frames, low-temperature storage protocols will be invaluable. Of course, ES cells can be cryopreserved, although weeks are required to establish viable cultures after thawing. In the present study, we found that both forms of NPCs could be stored using conventional equilibrium cooling or vitrification with rapid recovery of cultures in high yield. This capability allows the accumulation of cells for study with a significant savings in time and, ultimately, may be important in the context of cell-based therapy, in which large numbers of cells may be required to treat acute conditions over relatively short time frames.