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Helicobacter pylori anti-CagA antibodies: RESULTS Part 2

Asymptomatic subjects
Among the 119 asymptomatic subjects, 76 (64%) were found to be H pylori positive by ELISA. The sera from 40 of these subjects (randomly selected) were analyzed by immunoblotting. Table 3 compares the immunoblot results of H pylori antigens in symptomatic and asymptomatic subjects. Immunoblots revealed antibodies to CagA antigen in 26 (87%) symptomatic and 33 (83%) asymptomatic subjects. The frequencies of immunoreactive bands to VacA and to other antigens in the immunoblots of both groups were variable. The CIM was detected in 35 of 40 (87%) positive strips. Figure 1 shows representative immunoblot strips from H pylori-positive, asymptomatic subjects.

TABLE 3
Prevalence of antibodies to Helicobacter pylori antigens in immunoblots of symptomatic and asymptomatic subjects

Molecular weight of antigens (kDa)
Subject group 116 (CagA) 89 (VacA) 37 35 30 19.5
Symptomatic, n=30 (%) 26 (87) 21 (70) 21 (70) 19 (63) 27 (90) 19 (63)
Asymptomatic, n=40 (%) 33 (83) 26 (65) 30 (75) 30 (75) 39 (98) 32 (80)

Representative immunoblot strips from Helicobacter pylori-positive asymptomatic subjects
Figure 1) Representative immunoblot strips from Helicobacter pylori-positive asymptomatic subjects. N Negative control (normal human serum negative for antibodies to H pylori); P Positive control (inactivated human serum with immunoglobulin G antibodies to H pylori)

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