Females from these colonies between the ages of 30 and 66 days were housed (3-6 animals per cage) at the University of Maryland Central Animal Facility. Transgenic mice were genotyped using polymerase chain reaction (PCR)-based assays that have been previously described. Only wild-type mice and mice with homozygous deletion or overexpression were used in experiments. All animals were provided food and water for ad libitum consumption. Temperature was maintained at 22 ± 1°C and animals were subjected to 12L:12D cycles.
CD-1 mice were dosed beginning at 39 days old with 8-64 mg kg-1 day-1 of MXC or sesame oil (vehicle control). Bcl-2 overexpressing mice were divided into two groups for dosing, one group receiving 64 mg/kg MXC and the other receiving sesame oil. Their wild-type littermates were also divided into two groups: one group receiving 64 mg/kg MXC and the other group receiving sesame oil. Similarly, BaxKOs and their wild-type littermates were divided into two groups, with one receiving 64 mg/ kg MXC and the other receiving sesame oil.