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Methoxychlor-Induced Atresia: RESULTS(1)

RESULTS(1)

Effect of MXC on Atresia in CD-1 Mice

MXC caused a dose-dependent increase in the percentage of atretic antral follicles compared with controls at the 32 mg/kg dose (72% atretic follicles with MXC treatment compared with 57% atretic follicles with sesame oil treatment) and 64 mg/kg dose (87% atretic follicles with MXC treatment compared with 57% atretic follicles with sesame oil treatment) after 20 days of dosing (Fig. 1; n = 3-9, P s 0.001). In addition, mice treated with 64 mg/kg MXC had fewer antral follicles than those treated with sesame oil. The sesame oil-treated animals had a total of 1632 ± 192 antral follicles, whereas animals treated with 64 mg/kg MXC had a total number of 936 ± 136 antral follicles (n = 3-9, P s 0.01).

As an estimate of ovulation capacity, the number of CL in controls and MXC-treated ovaries were counted. The results indicate that the average number of CLs per ovary declined significantly with MXC treatment. There was an average of 9.5 ± 0.9 CL in the sesame oil-treatment group, 6.0 ± 0.7 in the 32 mg kg—1 day1 MXC-treatment group and 4.8 ± 0.9 in the 64 mg kg—1 day1 MXC treatment group (n = 3-7, P s 0.01). There was no overt toxicity nor any change in the weights of the animals at these doses of MXC.