Methoxychlor-Induced Atresia: RESULTS(4)

Effect of MXC on the Ovaries of Mice Deficient in Bax Protein

In wild-type mice treated with MXC (64 mg kg-1 day-1 for 20 days), there was a significant increase in the percentage of atretic follicles compared with sesame oil-treated counterparts (73% atretic follicles in the MXC-treated wild-type mice vs. 49% in the sesame oil-treated wild-type mice; Fig. 5; n = 3-5; P < 0.0001). In BaxKO mice, however, MXC was unable to increase the percentage of atretic follicles (31% atretic follicles in the MXC-treated BaxKO vs. 27% in the sesame oil-treated BaxKO mice; Fig. 5; n = 35; P = 0.5).

Although there was an increase in the percentage of atretic follicles in MXC-treated wild-type mice vs. sesame oil-treated wild-type mice, there was no significant change in the total number of antral follicles between MXC-treated wild-type mice and sesame oil-treated wild-type mice (n = 3-5). In contrast, there was a striking increase in the total number of atretic antral follicles between MXC-treated wild-type mice (1387 ±141) when compared with the MXC-treated BaxKO mice (480 ± 33; n = 3-5, P < 0.001).
Fig5Methoxychlor-Induced Atresia-5
FIG. 5. Effect of MXC on atresia in BaxKO mice. Bars, Means ± SEM; bars with different letters, significantly different from each other. (n = 35; P s 0.02).