Methoxychlor-Induced Atresia: RESULTS(5)

RESULTS(5)Effect of MXC on Estradiol, FSH, and LH Levels

After 20 days of dosing, there were no differences in estradiol levels between sesame oil-treated mice and mice dosed with 8, 16, 32, or 64 mg kg-1 day-1 MXC (Fig. 6A; n = 6-9, P = 0.74). FSH levels also did not differ between sesame oil-treated mice and mice treated with 8, 16, 32, or 64 mg kg-1 day-1 MXC (Fig. 6B; n = 6-9; P = 0.39). Finally, LH levels were unchanged between sesame oil-treated mice and those treated with 32 mg kg-1 day-1 MXC (Fig. 6C; n = 3; P = 0.2). my canadian neighbor pharmacy

Effect of MXC on ERa, ER/3, and FSH Receptor Levels

After 20 days of dosing, there were no significant differences in ERa or ERp protein levels between mice treated with sesame oil or mice treated with 8, 16, 32, or 64 mg kg—1 day—1 MXC (Fig. 7, A and B). In order to confirm these results, immunohistochemical analysis of ERa and ERp staining intensity was performed on ovarian sections from mice treated with sesame oil and 64 mg kg—1 day—1 MXC. Similarly, there were no differences in ERa or ERp levels between the sesame oil- and MXC-treated animals (data not shown). When ERp staining was analyzed in the follicles, there were no differences in staining intensity among the different follicle types. In the ERa-stained ovaries, there was no major staining in the follicles. Finally, there were no significant differences in the levels of FSHR mRNA between mice treated with sesame oil and 64 mg kg—1 day—1 MXC. The mean pixel density of the p-actin corrected values for FSHR in the sesame oil-treated ovaries was 1.1 ± 0.2, and for MXC-treated ovaries, it was 1.42 ± 0.2 (n = 3; P = 0.3).
Fig6Methoxychlor-Induced Atresia-6
FIG. 6. Effect of MXC on estradiol, FSH, and LH levels. Blood was collected during estrus after 20 days of dosing from sesame oil or 8, 16, 3,2 or 64 mg kg-1 day-1 MXC-treated mice. A) The levels of estradiol, (B) the levels of FSH, (C) the levels of LH. Bars, Mean ± SEM, n = 3-9.

Fig7Methoxychlor-Induced Atresia-7
FIG. 7. Effects of MXC on ERa and ERp protein levels. A) A graphical representation of data from four separate Western blot experiments measuring ovarian ERa protein levels. B) A graphical representation of four separate Western blots measuring ovarian ERp protein levels. Bars represent means ± SEM; n = 3-4.