News about Health (Part 77)

Helicobacter pylori anti-CagA antibodies: SUBJECTS AND METHOD Part 2

CLO test
One biopsy was placed into the CLOtest (Clia Waived, USA) and kept at room temperature. The results were recorded as specified by the manufacturer.

Biopsies for culture were dissected into small pieces using a sterile blade and then inoculated onto Colombia agar medium (Oxoid, United Kingdom), with 5% defibrinated sheep blood and selective supplements (H pylori-selective supplement, Oxoid). The cultures were incubated in anaerobic jars, in humidified, microaerophilic conditions, using CompyGen Gas pack (Oxoid) at 37°C for five days. The organisms were identified as H pylori by Gram staining, colony morphology, and urease, oxidase and catalase-posi-tive reactions.

Helicobacter pylori anti-CagA antibodies: SUBJECTS AND METHOD Part 1

Symptomatic subjects: Sixty-six patients attending the endoscopy unit at the Samatya Hospital, Istanbul, Turkey, from March to July 2000, with dyspepsia and abdominal pain as their main complaint, were selected randomly. The study group comprised 38 men and 28 women, 16 to 74 years of age (average age 45 years). All patients underwent endoscopy, and three biopsies were taken from the antral part of the stomach for CLO, culture and histology. A serum sample was obtained from each patient and stored at —20°C. Asymptomatic subjects: One hundred nineteen subjects (blood donors, school teachers and health workers), 20 to 65 years of age (average age 37 years), with no history of abdominal pain were selected randomly. A serum sample was obtained from each subject and stored at —20°C. A questionnaire was filled out for each subject.

Helicobacter pylori anti-CagA antibodies: Prevalence in symptomatic and asymptomatic subjects in Turkey

Helicobacter pyloriHelicobacter pylori colonize the stomachs of approximately 50% of the world’s population. The prevalence of infection ranges from 25% in developed countries to more than 90% in developing countries. In spite of this high prevalence, only a small percentage of infected individuals develop peptic ulcer disease, of which H pylori plays an important role in the pathogenesis. The cytotoxin-associated gene (cagA) and vacuolating cyto-toxin gene (vacA) are among the important factors in the pathogenesis of peptic ulcer disease. These genes encode for CagA and VacA proteins, which stimulate antibody formation in infected patients. The association of such antibodies with an increased risk of developing peptic ulcer diseases is controversial. Investigators from developed countries have found a significant association between the prevalence of CagA antibodies and duodenal ulcer. However, other studies, particularly those from developing countries, have shown no such association.

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