This study has shown that marmoset oocytes can be par-thenogenetically activated by electrical stimulation or ethanol treatment. Ninety-two percent of marmoset oocytes were activated by multiple electrical pulses. This is comparable to the rate of human oocyte activation (95%) using mannitol exposure and electrical stimulation. Human oocytes can be activated by ethanol at rates of 16%, similar to the 20% activation rate described here for marmoset oocytes using the same method.
Although puromycin can activate human oocytes at rates comparable to the electrical activation of marmoset oocytes reported here, puro-mycin compromises developmental potential. Thus, almost 90% of puromycin-treated oocytes do not cleave after activation, even in culture conditions suitable for human embryo development to the blastocyst stage. Electrical stimulation provides an activation mechanism that is an alternative to substances that have demonstrated the potential to disrupt cytokinesis and cell survival. antibiotic levaquin
Marmoset oocytes underwent four different types of development after activation, similar to those reported for human and mouse oocytes. The majority of ethanol-activated marmoset oocytes underwent immediate cleavage.