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Parthenogenetic Activation of Marmoset: MATERIALS AND METHODS(4)


Culture of Marmoset Oocytes andAssessment of Parthenogenetic Activation

After activation stimulus was applied, all oocytes were washed thoroughly and cultured singly in ~50-^l drops of MEM supplemented with 10% heat-inactivated female marmoset serum overlaid with paraffin oil to prevent evaporation. Oocytes were incubated at 37°C in a humidified atmosphere of 5% CO2 in air.

Oocytes were examined, using an inverted Olympus OMT-2 microscope fitted with Nomarski optics, 6 and 1820 h after activation stimulus, and daily thereafter. Pronu-clear formation, polar body extrusion, and cell number were assessed at each observation. Parthenogenetic embryos were either left in culture till developmental arrest, as determined by lack of cleavage for at least 24 h, or transferred at the 4-cell stage to recipient females. ventolin 100 mcg

Transfer of Parthenogenetic Marmoset Embryos to Recipient Marmosets

Cloprostenol was administered to recipient animals on the same day as donor animals. Recipient females were housed singly from the time of cloprostenol administration until 6 days after embryo transfer. Recipients received hCG (75 IU) at 1000 h, 8 days after cloprostenol, and a 0.3-ml blood sample was taken from the femoral vein. A second blood sample was taken 48 h later.