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Parthenogenetic Activation of Marmoset: MATERIALS AND METHODS(5)

Ovulation was confirmed by plasma progesterone levels over 10 ng/ml. Par-thenogenetic (n = 3) or biparental in vitro-fertilized (IVF) control (n = 2) embryos were transferred at the 4-cell stage to synchronized recipient female marmosets (n = 5) 3 days after hCG injection. Embryo transfers were carried out as previously described. Briefly, at midline laparotomy, the uterus was exteriorized and a hole was made in the uterine fundus using a 19-gauge needle. The embryo was introduced into the fundus in a pulled glass Pasteur pipette attached by rubber tubing to a micrometer syringe. The contents of the pipette (approximately 3 ^l) were gently expelled into the uterine lumen. Anesthesia and postoperative care were carried out as described previously. buy birth control online

In Vitro Fertilization of Marmoset Biparental Control Embryos

In vitro fertilization was carried out as previously described. Briefly, sperm were collected by epididy-mal dissection. Both oocytes and sperm were incubated in aMEM supplemented with 10 ^M dibutyryl cAMP, 10 caffeine, 6 mg/100 ml penicillin, 5 mg/100 ml streptomycin sulphate, and 10% heat-inactivated male marmoset serum. Oocytes were incubated for 9-11 h, and sperm were incubated for at least 3 h and up to 7-8 h before insemination.