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Parthenogenetic Activation of Marmoset: MATERIALS AND METHODS(7)

Whole-Body Perfusion Fixation of Recipient Female Marmosets

Recipients that had high (>50 ng/ml) progesterone until at least Day 26 were considered pregnant, and these animals were killed. The females were anesthetized with 1 ml of Saffan administered by i.m. injection. Once the animals were under general anesthesia, the peritoneum and thoracic cavity were opened. The ovaries were inspected for the presence of corpora lutea (CL). A 21-gauge butterfly catheter (Centaur) was inserted into the left ventricle of the heart and held in place with artery forceps, and the vena cava was cut posterior to the renal veins. The animal was perfused by delivering 200 ml heparinized (1000 IU/ml) PBS and then 200 ml 2.5% glutaraldehyde through the catheter. After perfusion, the uterus was dissected from the reproductive tract and stored in 2.5% glutaraldehyde until sectioning was carried out.

Sectioning and Staining of Marmoset Uteri

Sectioning was performed by the Department of Histo-pathology at the Royal Free Hospital School of Medicine (London, UK). Paraffin wax sections (3-^m thick) of perfused marmoset uteri were cut on the anterior-posterior axis, and every fifth section was mounted on a glass slide and stained with hematoxylin and eosin. Sections were inspected under brightfield microscopy, and relevant sections were photographed. buy cipro

Statistical Analyses

The differences in the proportions of oocytes undergoing each type of development after ethanol or electrical exposure were analyzed by ANOVA (CSS: Statistica; Statsoft U.K., Letchworth, UK).