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Parthenogenetic Activation of Marmoset: RESULTS(1)

RESULTS(1)

Oocyte Collection

A total of 122 oocytes were collected for this study, from 51 laparotomies. The average number of follicles per animal was 2.6, and the mean number of oocytes collected per animal was 2.4. buy ventolin inhalers

Visualization of Activation Events

Six hours after activation stimulus, some oocytes had undergone immediate cleavage (IC) to 2 cells. After 18-20 h, oocytes had either cleaved (IC), extruded a second polar body and formed one pronucleus (2PB,1PN), formed one pronucleus without extruding a second polar body (1PB,1PN), formed two pronuclei without extruding a second polar body (1PB,2PN), or failed to activate. These events occurred with the same timing after activation stimulus, regardless of whether ethanol or electrical stimulation was used.

Activation and Development of Marmoset Oocytes Exposed to Electrical Stimulation

Ninety-two percent (68 of 74) of marmoset oocytes were parthenogenetically activated after exposure to two series of six electrical pulses. The average cell number achieved in vitro was not significantly affected by the type of development (1PN,2PB; 2PN,1PB; 1PN,1PB, or iC) after activation (p < 0.05; Table 1). Nor was there a significant difference between the in vitro development of haploid (1PN,2PB or IC) and diploid (2PN,1PB or 1PN,1PB) par-thenogenones in this treatment group (p < 0.05). Overall, the average cell number (± SEM) of electrically stimulated parthenogenones was 4.0 ± 0.3.

TABLE 1.

Maximum cell number reached in vitro Type of development after activation (%)
IC 1PN,2PB 2PN,1PB 1PN,1PB Total
1-cell 2 2
2-cell 4 6 5 3 18
3-cell 1 5 6 12
4-cell 2 9 3 3 17
5-cell 1 1 2
6-cell 4 1 1 6
7-cell 1 1
8-cell 2 2 6 10
Oocytes activated (%) 9 30 22 7 68/74
Average cell number (±SEM) 3.9 (± 0.8) 3.9 0.3) 4.5 (± 0.5) 3.4 (± 0.6) 4.0 (± 0.3)