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Parthenogenetic Activation of Marmoset: RESULTS(3)

RESULTS(3)

The levels of progesterone in the peripheral plasma of both animals (345W and 457W) that received biparental IVF embryos rose steadily to about 100-120 ng/ml and remained high, typical of progesterone profiles of pregnancy (Fig. 1A). Of the three animals that received partheno-genetic embryos, two animals (469W and 491W) had progesterone profiles resembling those of pregnant marmosets (Fig. 1C), and one recipient (323W) had a progesterone profile typical of a nonpregnant animal (Fig. 1E). Continued high progesterone levels in animals 469W and 491W for 33 days after ovulation would suggest that the CL was maintained, and this was confirmed visually when these recipient marmosets were killed.

CG Profiles of Recipient Marmosets

Normal levels of CG, which strongly cross-reacts with LH, have also been previously described by Hearn et al.. The natural LH surge and/or administration of exogenous hCG, used to synchronize recipient animals, causes LH/CG to rise sharply around the day of ovulation. If pregnancy is not established, LH/CG levels drop to baseline levels (<40 mIU/ml) and remain low until just before the following ovulation. If pregnancy is established, LH/ CG (~50 mIU/ml) can be detected in the peripheral plasma from Day 16-20 and can rise to levels of 1000 mIU/ml by Day 60 of gestation. buy flovent inhaler

Animals that received biparental IVF embryos had LH/ CG profiles typical of pregnancy (Fig. 1B). All animals that received parthenogenetic embryos had LH/CG profiles resembling those of nonpregnant animals (Fig. 1, D and F).
Fig1Parthenogenetic Activation
FIG. 1. Progesterone and LH/CG profiles for recipient animals after transfer of either biparental IVF embryos (A, B) or parthenogenetic embryos (C-F). Profiles of IVF embryo recipient animals (345W and 457W) are shown in A (progesterone) and B (CG). The profiles for parthenogenetic recipient animals (469W and 491W) are shown in C (progesterone) and D (CG), and the profile for animal 323W (nonpregnant) is shown in E (progesterone) and F (CG).