Rat Testis Motor Proteins Associated with Spermatid Translocation: DISCUSSION(1)


In the study reported here, we determined sites in the seminiferous epithelium that are immunoreactive with antibodies to the intermediate chain of cytoplasmic dynein and kinesin-II. Cheap Diskus Advair

Our results provide a morphological correlate for the high levels of cytoplasmic dynein detected previously in testis and in Sertoli cells. The data indicate that an isotype of cytoplasmic dynein may be present in Sertoli cell regions associated with apical crypts containing attached elongate spermatids. They also support the conclusion that a form of cytoplasmic dynein is most likely related to, but not entirely restricted to, unique intercellular attachment plaques that occur in these regions and that have been proposed to translocate spermatids in the seminiferous epithelium.

A number of observations indicate that the IC74 antibody used in this study is reasonably specific for the intermediate chain of cytoplasmic dynein when used to probe rat testis. On immunoblots of isolated seminiferous epithelium, the antibody reacted predominantly with a single band at the appropriate molecular mass for the intermediate chain of cytoplasmic dynein (74 kDa). Moreover, a specific pattern of fluorescence was not observed when the antibody was replaced with normal mouse IgG, nor was the pattern observed in the second antibody control or in the control for autofluorescence.