In Sertoli cells, intense staining with the IC74 antibody was concentrated in columnar regions of the cell surrounding apical crypts containing attached spermatids. Although results from immunofluorescence and from experiments with gelsolin suggest an association of the immunoreactive protein with unique intercellular adhesion plaques related to spermatid heads, the staining pattern in fixed frozen sections of rat testis indicates that the protein is certainly not restricted to these areas. It occurs diffusely elsewhere in the cytoplasm and in linear tracts along structures, possibly cis-ternae of endoplasmic reticulum, projecting apically from regions containing the plaques. ventolin 100 mcg
There is now a growing body of evidence consistent with the microtubule-based hypothesis of spermatid translocation. In Sertoli cells, microtubules are arranged parallel to the axis of translocation and are morphologically related to the junction plaques attached to spermatid heads. In vitro, exogenous microtubules bind to the endoplasmic reticulum component of the plaques in a nucleotide-dependent fashion, and in motility assays, the junction plaques support microtubule movement. Results presented here demonstrate that Sertoli cell regions surrounding apical crypts and containing the unique intercellular adhesion junctions proposed to be related to spermatid translocation are strongly reactive with an immunological probe for the 74-kDa cytoplasmic dynein subunit.