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Spectroscopic approach to capillary-alveolar membrane damage: PATIENTS AND METHODS (2)

Lung injury

Bronchial washing samples were collected by advancing the inline suction catheter through the endotracheal tube until a slight resistance was felt, usually at a distance of 35 to 40 cm. At this distance, the tip of the catheter would be expected to lie in a segmental or subsegmental bronchus. A volume of 10 to 20 mL of normal saline was then flushed down the catheter. After several respiratory cycles, the catheter was aspirated using 50 to 75 mmHg of negative pressure and then withdrawn. Aspirated fluid was collected in a Lukens trap (Sherwood Medical, St Louis, Missouri) with the usual yield approximately 3 to 4 mL. Once obtained, the samples were refrigerated immediately and transported to the Institute for Biodiagnostics, Winnipeg, Manitoba for processing within 24 h. The IR spectrum of the bronchial washing with HES did not change over 24 h. Bronchial washing fluid is the aspiration of small amounts of saline and secretions from large airways. The washing can be performed quickly and easily in an intubated patient, and no adverse clinical effects of sample collection have been documented. Indeed, the method of bronchial washing is the standard pulmonary cleaning method in many intensive care units. buy prednisone

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