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Spectroscopic approach to capillary-alveolar membrane damage: RESULTS AND DISCUSSION (4)

The patient died four days postinjury. Autopsy histology showed acute diffuse alveolar damage with hyaline membranes and superimposed bronchopneumonia (Figure 3). Frozen autopsy tissue was prepared for IR microscopic examination as 10 |u,m thin unstained sections. The thin section was placed onto a calcium fluoride window and allowed to dry. A spectrometer equipped with a 15x objective microscope (Bruker Optik, Karlsruhe, Germany) was used for IR pathological examination. Two randomly selected areas from within the section, each 300×300 |u,m , were investigated by IR microspectroscopy. For each of the selected areas, 100 individual spectra at a resolution of 4 cm-1, with each spectrum covering an area of 30×30 |u,m2, were recorded. Colour images of the distribution of PENTASPAN within the examined tissue sections are illustrated in Figure 4 and were generated based on the intensity of the PENTAS-PAN absorption band at 1030 cm-1. The false colour code in Figure 4 (right) ranges from white (no PENTASPAN) through green (traces of PENTASPAN) and yellow (small amounts of PENTASPAN) to red (large amounts of PENTASPAN). While both sections contain PENTASPAN with diffuse patterns, the section in Figure 4 (middle) clearly demonstrates a severe tissue injury. The spectroscopic images provide pathological evidence of capillary-alveolar membrane damage and site-to-site variability of PENTAS-PAN within the injured tissue sections. Buy Asthma Inhalers Online

Figure 3. Spectroscopic approach
Figure 3) Microscopic section from autopsy lung tissue, showing acute diffuse alveolar damage with hyaline membranes (small arrows) and superimposed bronchopneumonia (large arrow. Leitz Diaplan microscope (Leica, Heidelberg, Germany) (10x magnification)

Figure 4. Spectroscopic approach
Figure 4) Two-dimensional microspectroscopic images (based on PENTASPAN distribution) of two 10 m thick lung tissue sections of 300×300^m2. The false colour-code ranges from white (no PENTASPAN) through green (traces of PENTASPAN) and yellow (small amounts of PENTASPAN) to red (large amounts of PENTASPAN)

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