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Stage-Dependent Accumulation of Cadmium: MATERIALS AND METHODS(3)

MATERIALS AND METHODS(3)Tissue Cd Concentrations

To determine actual Cd levels, staged testicular tissues and liver, kidney, heart, and muscle were collected from untreated controls and from animals injected with CdCl2 (5 mg/kg body weight) 3 days before death as described above (3 per group); samples were stored at -70°C until analysis. Samples (200 mg) were weighed into acid-cleaned polytet-rafluoroethylene vials and digested at 70°C in trace metal-grade concentrated nitric acid (Fisher, Pittsburgh, PA), which was then evaporated to dryness at 85°C. The residue was reconstituted in 3% nitric acid and filtered (0.45 ^M). buy cipro

Cd was assayed on a Perkin-Elmer 5100 PC Atomic Absorption Spectrophotometer with Zeeman background correction equipped with a heated graphite atomizer furnace and programmer, an electrodeless discharge lamp, and an argon purge gas (Perkin-Elmer, Norwalk, CT). A matrix modifier (0.6 mg/ml Mg and 0.4 ml/ml Pd in 0.2% nitric acid) was added to the samples to stabilize Cd during the furnace char step. The linear range was 0-11 ^g/L and the detection limit was 0.15 ^g/L. For tissues measuring > 11 ^g/L, samples were diluted 10-fold in 3% nitric acid. Certified atomic absorption Cd standards were from Perkin-Elmer. Values were expressed as ^g Cd/g tissue.