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Tag: follicle

Methoxychlor-Induced Atresia: MATERIALS AND METHODS(11)

A standard curve was generated from five serial dilutions of one of the samples, thus allowing analysis of the amount of cDNA in the exponential phase. FSHR primer sequences were: (forward) 5′-AGCAAGTTTGGCTGTTATGAG G-3′, (reverse) 5′-GTTCTGGATGATTTAGAGG-3′. An initial incubation of 95°C Read More

Methoxychlor-Induced Atresia: MATERIALS AND METHODS(6)

In some experiments, sections were processed for immunolocalization of ERa and ERp using a commercially available antibody against ERa (1:80 dilution; Santa Cruz Biotechnology,) or ERp (1:360 dilution; Zymed Laboratories, San Franscisco, CA). The secondary antibody and visualization reagents were Read More

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