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Tag: follicle

Vascular Remodeling and Angiogenesis: RESULTS(3)

Kinetic Studies of Intramuscular Ovarian Transplantation The subcutaneous grafts showed extensive damage during the first days after transplantation. Therefore, the intramuscular grafts were assessed at different time periods, from 1 to 31 days posttransplantation (Fig. 3). Half ovaries were transplanted Read More

Vascular Remodeling and Angiogenesis: RESULTS(1)

Subcutaneous Ovarian Transplantation Ovaries of 15-day-old Wistar rats contain primordial, primary, and small antral follicles (Fig. 1A). The GSL-1 staining represents staining of endothelial cells (e.g., Fig. 1B) and aSMA staining represents SMC and pericytes (e.g., Fig. 1C). Subcutaneous half ovarian Read More

Vascular Remodeling and Angiogenesis: MATERIALS AND METHODS(6)

Follicle Examination The eosin-hematoxylin-stained slides were used for follicle counts. The follicles were counted in all the detected grafts (Table 1). Both viable and atretic follicles were counted. Follicles were considered atretic if more than 1% pyknotic nuclei were found Read More

Vascular Remodeling and Angiogenesis: MATERIALS AND METHODS(4)

The concentration maps were used for derivation of vascular permeability (apparent permeability surface area product, APSMRI), namely the rate of contrast accumulation, derived by linear regression of the first 10 min of postcontrast agent administration. Only pixels with significant regression Read More

Vascular Remodeling and Angiogenesis: MATERIALS AND METHODS(3)

BSA-based macromolecular contrast material, biotin2-BSA-gadolini-um-DTPA23 (biotin-BSA-GdDTPA; about 82 kDa) was prepared as reported previously and bolus injected through a tail vein catheter (12.4 mg/mouse in 0.2 ml). A series of precontrast spin echo images, with repetition time (TR) values of Read More

Vascular Remodeling and Angiogenesis: MATERIALS AND METHODS(2)

In all transplantations, one ovary piece was transplanted into each mouse. In the subcutaneous transplantation, half ovaries were transplanted into 13 mice (Table 1). The ovaries were retrieved between Days 1 and 24 after transplantation. In the intramuscular ovarian transplantation, Read More

Vascular Remodeling and Angiogenesis: MATERIALS AND METHODS(1)

Ovary Retrieval and Transplantation All animal experiments were approved by the Institutional Animal Care and Use Committee. Immature 15-day-old Wistar rats were killed using CO2 and subsequent cervical dislocation, and ovaries were collected. The ovaries were cleaned from fat in Read More

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