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Tag: gamete biology

Immunohistochemical Profiling of Germ Cells: DISCUSSION(9)

In summary, gonocytes are a population of single, round cells characterized as OCT4pos/C-KITpos/MAGE-A4neg. Intermediate cells usually occur in pairs and are characterized as C-KITneg/MAGE-A4neg/OCT4low/neg. Both these cell populations are PCNA-positive and, therefore, would be grouped together as M prospermatogonia according Read More

Immunohistochemical Profiling of Germ Cells: DISCUSSION(6)

Wartenberg classified the fetal germ cell population into M prosper-matogonia (proliferating), T1 spermatogonia (nonproliferating), and T2 prospermatogonia (those T spermatogonia that had resumed mitotic activity). In follow-up studies, we have been examining the mitotic activity of human fetal germ cells Read More

Immunohistochemical Profiling of Germ Cells: DISCUSSION(4)

In the present study, consistent with these observations, we noted that germ cells with distinctive morphologies (small, round, and single vs. larger, peripheral groups) were present in second-trimester samples. OCT4 (POU5F1 ), a transcription factor that is expressed in pluripotent Read More

Immunohistochemical Profiling of Germ Cells: DISCUSSION(1)

In the present study, functional Sertoli cells were identified by immunostaining for MIS. In samples dated as 4964 days of gestation, the degree to which the MIS-positive cells were organized into ‘‘cords’’ varied considerably between samples. In all cases, the Read More

Immunohistochemical Profiling of Germ Cells: RESULTS(4)http://health-and-you-life.com/immunohistochemical-profiling-of-germ-cells-9.html

Cells in population 3 were larger than those in the other two populations. Because of the presence of more cytoplasm, their nuclei did not contain such a prominent nucleolus as populations 1 or 2, and these cells were typically found Read More

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