Tag: gamete biology

In summary, gonocytes are a population of single, round cells characterized as OCT4pos/C-KITpos/MAGE-A4neg. Intermediate cells usually occur in pairs and are characterized as C-KITneg/MAGE-A4neg/OCT4low/neg. Both these cell populations are PCNA-positive and, therefore, would be grouped together as M prospermatogonia according Read More

In our studies on the fetal testes, we have never found a germ cell that coexpressed C-KIT and MAGE-A4, and our preliminary observations (unpublished) suggest that although C-KITpos and MAGE-A4pos CIS cells can be detected, the two proteins are not Read More

A comparison between the patterns of protein expression seen in the germ cell subpopulations identified in the current study and the patterns of protein expression reported for CIS cells appears to provide further data relevant to determining the cells that Read More

Wartenberg classified the fetal germ cell population into M prosper-matogonia (proliferating), T1 spermatogonia (nonproliferating), and T2 prospermatogonia (those T spermatogonia that had resumed mitotic activity). In follow-up studies, we have been examining the mitotic activity of human fetal germ cells Read More

In mice, expression of Oct4 in germ cells has been studied using a transgene in which expression of green fluorescent protein was driven by an 18-kilobase fragment encompassing the Oct-4 gene. In the fetal testes, OCT4-posi-tive germ cells were mitotically Read More

In the present study, consistent with these observations, we noted that germ cells with distinctive morphologies (small, round, and single vs. larger, peripheral groups) were present in second-trimester samples. OCT4 (POU5F1 ), a transcription factor that is expressed in pluripotent Read More

The patterns of expression of C-KIT and PCTAIRE-1 were similar, with a reduction in the proportion of positive germ cells in 17- to 19-wk samples compared to less mature samples. Mice that lack a functional C-KIT receptor contain less germ Read More

Results from studies in rodent are in broad agreement with those in humans and indicate that functional maturation of Leydig cells occurs after formation of MIS-positive seminiferous cords and that AR immunoexpression is not detectable in the nuclei of Sertoli Read More

In the present study, functional Sertoli cells were identified by immunostaining for MIS. In samples dated as 4964 days of gestation, the degree to which the MIS-positive cells were organized into ‘‘cords’’ varied considerably between samples. In all cases, the Read More

Cells in population 3 were larger than those in the other two populations. Because of the presence of more cytoplasm, their nuclei did not contain such a prominent nucleolus as populations 1 or 2, and these cells were typically found Read More