Loading

wait a moment

Tag: monkey

Differentiation of Monkey Embryonic Stem Cells: DISCUSSION(3)

Precursors of other cell lineages or undifferentiated phenotypes likely compose the majority of the cell population within the early EBs used in NPC generation. Therefore, a lower rate of neuronal differentiation would be expected after expansion and differentiation. This is Read More

Differentiation of Monkey Embryonic Stem Cells: DISCUSSION(1)

With the derivation of hES cells and the demonstration of their pluripotency in vivo, cell-based therapy of human degenerative diseases becomes a realistic possibility. However, little current evidence shows that these cells will efficiently and permanently ameliorate disease conditions without Read More

Differentiation of Monkey Embryonic Stem Cells: RESULTS(4)

Because NPCs represent a convenient starting point for in vitro neural differentiation, a conventional DMSO-based, controlled-rate cooling program was applied to the adherent cells described previously with subsequent storage in liquid nitrogen. On thawing, 75.4% ± 8.7% (four replicates) of Read More

Differentiation of Monkey Embryonic Stem Cells: MATERIALS AND METHODS(4)

The primary antibodies used were against SSEA-1, SSEA-3, SSEA-4, TRA-1-60, and TRA-1-81 (mouse monoclonal, kindly provided by Dr. Peter W. Andrews, University of Sheffield, Sheffield, U.K.), Oct-4 (Santa Cruz Biotechnology, Santa Cruz, CA), a-fetoprotein (mouse monoclonal, 1:500; Sigma), cardiac troponin Read More

Pages: 1 2 Next