The aim of this study was to investigate the angiogenic processes that occur after ovary xenotransplantation in order to find a way to protect ovarian grafts from posttransplantation damage. The experimental model of rat ovaries transplanted into immunodeficient mice was used for monitoring vascular remodeling by magnetic resonance imaging (MRI) and histology. The rat ovarian cycle is well defined and has been studied extensively, including some MRI studies on ovarian angiogenesis, while the nude mice provide a good model for MRI studies of angiogenesis of tumor xenografts. The ovaries were transplanted into two sites that represent tissues abundantly (intramuscular) or poorly (subcutaneous) vascularized.
Significant ovarian damage was observed in both cases within the first 24 h after transplantation. However, while the intramuscular grafts recovered, most of the subcutaneous grafts remained impaired and necrotic. Regions with high pericyte distribution showed improved follicular integrity relative to regions devoid of pericytes. As previously shown in other systems, these perivascular cells are important for vascular stabilization and maintenance. generic cialis canadian pharmacy
These results suggest that interventions aimed at improving ovarian transplantation should be focused on the establishment of perivascular support as early as possible after the implantation.