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Vascular Remodeling and Angiogenesis: MATERIALS AND METHODS(4)

The concentration maps were used for derivation of vascular permeability (apparent permeability surface area product, APSMRI), namely the rate of contrast accumulation, derived by linear regression of the first 10 min of postcontrast agent administration. Only pixels with significant regression (r > 0.9) were considered. Data are presented using a color scale for APSMRI values between 0 and 0.8 ^M/min, overlaid on a gray-scale anatomical image. Mean changes in APSMRI were calculated from selected regions of interest, mainly the graft and the adjacent muscle.

Contrast Agent Distribution in Intact Rat Ovaries

Rats were anesthetized by intraperitoneal administration of assival (Biogal; Teva Pharmaceutical Industries, Debrecen, Hungary), 125 mg/kg and ketamine (Fort Dodge Laboratories), 6.25 mg/kg. The contrast agent was applied to 15-70-day-old Wistar rats (via the tail vein or the heart). The animals were killed after 45 min in order to mimic the MRI procedure. Ovaries were retrieved for histological analysis after fixation. starlix 60 mg

Histology

Samples from the subcutaneous transplantations included the skin with the attached graft, while the grafts from the intramuscular transplantations were retrieved with the surrounding muscle as marked by the suture. The samples were placed overnight in Carnoy fixative solution (6:3:1 ethanol: chloroform:acetic acid), transferred into 70% ethanol, and stored at room temperature until processing. Fixed tissues were embedded in paraffin blocks and sectioned serially at 4-^m thickness.