Subcutaneous Ovarian Transplantation
Ovaries of 15-day-old Wistar rats contain primordial, primary, and small antral follicles (Fig. 1A). The GSL-1 staining represents staining of endothelial cells (e.g., Fig. 1B) and aSMA staining represents SMC and pericytes (e.g., Fig. 1C).
Subcutaneous half ovarian grafts were examined at various time points during the first days after transplantation (Fig. 1, D-L). Out of the 13 grafts, only 6 provided identifiable follicles within the ovarian sections (Table 1). A gradual decrease in the overall preservation (follicle state, vascular integrity, and necrosis) of the ovarian grafts was observed. micronase dosage
Damage at the center of the graft was detected 2 days after transplantation (Fig. 1D). Damage was also observed in blood vessels, where pericytes were the first to be affected, as is indicated by reduction in the aSMA staining (Fig. 1F). After 6 days, the damage expanded and only follicles at the graft periphery, located away from the necrotic center, survived (Fig. 1, G-I). Spatial correlation was observed between surviving follicles and maintenance of aSMA-positive cells (Fig. 1I). After 11 days, most of the ovary was necrotic (Fig. 1, J-L). The condition of the ovary remained poor 24 days after transplantation (even though a few follicles could be observed, most of them were necrotic). It seems that, during the first days after subcutaneous transplantation, extensive irreversible damage to the graft occurred.
FIG. 1. Ovaries of 15-day-old Wistar rats prior to and at various time points after subcutaneous transplantation in CD-1 nude mice. A-C) Ovaries prior to transplantation. D-F) Ovaries 2 days after transplantation. G-I) Ovaries 6 days after transplantation and (J-L) 11 days after transplantation. Left column: eosin hematoxylin staining; center column: endothelial cells staining (GSL-1) positive stain in brown; right column: smooth muscle cells and pericytes staining (aSMA) positive stain in red. Bar = 100 ^m.