Kinetic Studies of Intramuscular Ovarian Transplantation
The subcutaneous grafts showed extensive damage during the first days after transplantation. Therefore, the intramuscular grafts were assessed at different time periods, from 1 to 31 days posttransplantation (Fig. 3). Half ovaries were transplanted into the gluteus superficialis at the hind limb. Of the 31 grafts, 26 were detected (Table 1). One of the ovaries retrieved 24 h after transplantation was found to be disconnected from the muscle (Fig. 3, A-C). In that ovary, both the follicles and the vasculature were extensively damaged. Similar to the subcutaneous transplants, at this early time point after transplantation, damage was also detected in ovaries that were in close contact with the muscular tissue. buy glucovance
Nevertheless, in this case, survival of primordial follicles along with some mature blood vessels was observed (Fig. 3, D-I). The damage 3 days after transplantation was limited to the center of the grafts, while at the periphery, viable follicles and intact blood vessels were observed (Fig. 3, J-L). Beyond 6 days after the transplantation, the grafts seemed to recover and resembled normal ovaries (Fig. 3, M-R). Follicles at progressive developmental stages, ranging from primordial to antral, were detected.
FIG. 3. Chronological changes in the transplanted ovary following intramuscular transplantation. Half ovaries taken from 15-day-old Wistar rats were transplanted into the gluteus superficialis muscle at the hind limb of CD-1 nude mice and were retrieved after different time intervals. A C) An ovary that was found to be disconnected (N.C.) from the muscle 1 day after transplantation (n = 1). D-F) A connected graft 1 day after transplantation (n = 6). The marked areas are magnified in G-I; primordial follicles are marked by arrowheads. J-L) Grafts that were retrieved 3 days after transplantation (n = 5), (M-O) 7 days after transplantation (n = 5), and (PR) 28-31 days after transplantation (n = 7). Left column: eosin hematoxylin staining; center column: endothelial cells staining (GSL-1) positive stain in brown; right column: smooth muscle cells and pericytes staining (aSMA) positive stain in red. Bars in A-F and J-R = 100 ^m; bars in G-I = 50 ^m.