Dividing the intramuscular grafts into three groups according to the time after transplantation reveals that, during the short time periods of 1-3 days after transplantation, the ovaries scored —7.7 (n = 11), after 6-7 days, the score was 25.5 (n =;8), and at 28-31 days, the score was 8.0 (n = 7; Fig. 5). This scoring method reflects ovarian graft survival and was the lowest in the subcutaneous grafts throughout the 2-24 days after transplantation. The state of the intramuscular grafts was suboptimal during the first 3 days after transplantation; however, it improved after 6-7 days. After a period of about a month, a decrease in the follicular state was observed, although the grafts remained viable. The number of the small primordial follicles per square millimeter in the intramuscular site remained similar during the entire period examined (Fig. 4, D-F). canadian health & care mall
Intramuscular Ovary Transplantation: Angiogenesis
In order to detect and evaluate functional blood vessels in the graft and in the adjacent muscle, a high molecular weight contrast agent, biotin-Gd-DTPA-BSA, was administered through the tail vein. In addition to showing the functionality of the vessels, its leakage is also a marker for vascular permeability. The contrast agent was followed by dynamic contrast-enhanced MRI at 28-35 days after transplantation. After administration, the contrast agent was immediately detected in large blood vessels (Fig. 6B) and subsequently extravasated in the ovarian implant.